To isolate alkaloids from plants, the dried and powdered plant material is extracted with pet ether (or hexane, colemans etc.) first. This removes fats, oils, terpenes, waxes etc. This extract is discarded.

The material is now subjected to an alcohol extraction, eg with methanol or ethanol. The extract is evaporated to leave the crude alkaloids mixture.

This extract is partitioned between an diluted aq. tartaric acid solution and ethyl acetate. Other acids like citric acid can be used, and other solvents may substitute here. The ethyl acetate layer contains neutral and weakly basic alkaloids. Evaporate the solvent to isolate them.

The aq. layer is neutralised with NH3 or Na2CO3 and again extracted with ethyl acetate. The organic layer now contains basic alkaloids, while the aq. layer contains quarternary ammonium ions.

Many alkaloids can be isolated directly from the alkoholic extract by chromatographic methods. This is a separation which works well for tropane alkaloids (atropine, cocaine, scopolamine).

https://erowid.org/archive/rhodium/chemistry/alkastract.html

The alcohol extract is fractionated by column chromatography on SiO2, solvent chloroform followed by chloroform with rising methanol content. This separates lipids and terpenes from the crude alkaloid fraction. The alkaloid fraction is again chromatographed (SiO2; CHCl3 : MeOH = 10:1) to isolate the pure alkaloids.

Alkaloids containing basic amines can be selectively extracted using a modified version of

the classic ‘‘acid–base shakeout’’ method. Mineral acids and strong bases should be avoided

in extracting alkaloids (and plant material in general) because of the risk of artifact formation.

The alkaloid extraction scheme outlined employs tartaric acid and Na2CO3 to modify the pH

during partitioning.

 First, the pH is adjusted to at least two units below the pKa values of the basic amines

present, causing them to form salts, and thus to partition into the aqueous layer (along

with the polar constituents, including quaternary amines and alkaloid N-oxides). The

nonpolar constituents’ partition into the organic layer.

 After removal of the organic layer, the aqueous layer is made basic, producing the

free-base form of the basic alkaloids.

 Upon partitioning with fresh organic solvent, these alkaloids favor the organic layer,

producing an almost pure alkaloid fraction, with the exception of the N-oxides and

quaternary amines, that would remain in the aqueous layer.

 Alkaloids can also be extracted with 10%-acetic acid in EtOH, followed by

concentration under vacuum to one-quarter the original volume and precipitation of

the crude alkaloid fraction by drop wise addition of NH4OH.

 Alternatively, the plant material may first be wetted with a dilute base (NaHCO3,

Na2CO3, or CaCO3 solution), followed by percolation or maceration with nonpolar

organic solvent.

General procedure to obtain alkaloid extracts from crude plant material.

Since I read that only alkaloids are desired, extract the leaves with acidic methanol.

After that, ion exchange columns are useful to both remove the neutral compounds and start purifying the alkaloids.

For some examples, please see:

http://www.teledyneisco.com/Documents/Liquid%20Chromatography/Application%20Notes/Purification%20of%20Alkaloids%20with%20RediSep%20Rf%20SCX%20Columns%20App%20Note.pdf#search=alkaloids

http://www.teledyneisco.com/Documents/Liquid%20Chromatography/Application%20Notes/Hints%20for%20Strong%20Ion%20Exchange%20Resins%20App%20Note.pdf#search=alkaloids

http://www.teledyneisco.com/Documents/Liquid%20Chromatography/Posters/Alkaloid%20Purification%20Strategies%20Using%20Flash%20Chromatography%20Poster.pdf#search=alkaloids

How can yield be improved upon. ....I observed that low yield when first  method was adopted. 

I have a suggestion. Use methanol for your extraction. After evaporation of methanol, you should use acidic solution such as TFA for extraction of alkaloids from methanolic extraction plates. Again evaporate acidic solution and use chloroform for the extraction of acidic plate’s extraction.

Extraction is based on the isolation of alkaloids by soaking plant leaves in sodium hydroxide solution. And then filtered with the addition of chloroform to rinsing and then followed the separation of the organic layer with the separation of the separation, with the evaporation of chloroform finally get the alkaloids in raw form .

I have a suggestion, but I think it is expensive. I suggest the (CO2 super critical extraction technique).

I agree with Dr. Mustafa Albayati .

acid/base extraction method is most widely used method to seperate alkaloids from non-alkaloids. It requires acid and a base and chloroform extraction finally to seperate the alkaloids. Its easy and effective one

Can we isolate alkaloids from curculigo plant??

I totally agree with Dr. Mushtag Ahmed

All the above-mentioned are correct but first try on a minute amount to see how it walks before going with the whole plant material. All lies on your ability to effectively practice in the lab

I agree with Prof Mustafa Al-Hadeethi

I agree with Dr. Mustafa R. Al-Hadeethi, that it is true that the method is rather expensive, but effective and maintains the components of the plant.