Gourav Chandan

3 Questions 8 Answers 0 Followers

Questions related from Gourav Chandan

How can we analyze cancer markers proteins in Co culture assay (PBMCs and Cancer cell)using WESTERN BLOTTING?

We are checking cancer cell death by activated PBMCs in a co-culture system. We specifically want to analyze the markers (Caspases, PI3K/mTor/AKT, BAX BCL2 and several other markers) using...

31 January 2020 7,835 2 View

Which cells (Adherent or suspension cells) produce more ROS using DCF-DA assay to determine the intra-cellular ROS production?

we are working on different model system including leucocytes and cancer cell cultures to determine the intra-cellular ROS production. And does cells incubation for 24hrs or 48hrs gives you...

06 October 2016 2,830 6 View

Hello everyone. can anyone tell the link between PBMC and immunosuppression ?

im working on immunomodulation .The phtyochemical analysis shows the plant is rich in phenols especially flavonoids, tanins and terpenioids. . As im performing some experiment with PBMC as model...

22 July 2016 7,128 5 View