According to the article from Siddiqui et al. (2016) entitled "ISOPRENALINE: A TOOL FOR INDUCING MYOCARDIAL INFARCTION IN EXPERIMENTAL ANIMALS" published in the International Journal of Pharmacy 6(2): 138-144, tehere are several ways to induce myocaridal infarction using isoprenaline/isoproterenol, which vary in dose, administration route and exposure time:
• Low doses of isoproterenol (0.3–6 mg/kg body weight) administered acutely or repeatedly during 1–3 weeks
• Medium doses of isoproterenol (10–85 mg/kg body weight) applied in a single dose
• High doses of isoproterenol (150–300 mg/kg body weight) applied in a single dose or in two consecutive doses.
Moreover, searching the literature, I realized that are still other protocols, with the exception of the mentioned above.
Can any research collegue with experience in this model of myocardial injury justify which protocol is better?
Thanks in advance,
Patrick Türck
PhD in Physiology
ٍSeveral mechanisms proposed to explain the isoproterenol-induced myocardial harm, one might say: an unbalance between oxygen supply to and demand from cardiomyocytes inwardly, which is related to myocardial hyperfunction due to increase both in chronotropism and inotropism as well as to hypotension in the coronary bed [4]. Secondly, it is also claimed that there is an elevation of Ca++ overcharge inside the cell [5]. In addition, that ion is related to the activation of the adenylate cyclase enzyme and the depletion of ATP levels on the course of the events [6]. Eventually, there is an oxidative stress augmentation because of several metabolic products originated from isoproterenol, not to mention free radicals genesis
for more information see this link Article Experimental model of myocardial infarction induced by isopr...
regards
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