We are trying to find to detect naive T cells in the peripheral blood using flow cytometry. I would be happy to if anyone could help me and let me know if this is basically possible.
It is possible by using CCR7, CD28, CD95, CD62L markers. The % largely varies from person to person based on their age and their pathogen/vaccine exposure history. You just need to test a panel of samples to get your own data.
You may find this useful
In summary, naïve T cells are CD45RA+CD45RO-CCR7+CD62L+, central memory T cells are CD45RA-CD45RO+CCR7+CD62L+, effector memory T cells are CD45RA-CD45RO+CCR7-CD62L-, and effector cells are CD45RA+CD45RO-CCR7-CD62L-. Thus, CD3, CD4, CD8, CD45RA or CD45RO, and CCR7 or CD62L or CD27, are a great starting point for designing flow cytometry panels for the assessment of T cells present in human PBMC. {source: Basic markers of T cell populations in human PBMC
Posted on November 27, 2012 by Andrea]
Central memory and effector memory T cell subsets: function, generation, and maintenance. Sallusto F, Geginat J, Lanzavecchia A. Annu Rev Immunol. 2004;22:745-63.
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1 Recommendation
Percy Schröttner
Universitätsklinikum Carl Gustav Carus Dresden
Thank you so much for your help !
Kind regards,
Percy Schröttner
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Antonio Lapenna
Università Campus Bio-Medico di Roma
CD4 naive T cells can be detected as CD31+.
Look also publicatio attached.
Antonio
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