Hi. I am trying the egg albumin denaturalization method at 660 nm. However, the absorbance in diclofenac is too low (=0.050) when the diclofenac standard is = 1000 uL/mg. This is the method and 0 for the control.

The reaction mixture (5 mL) consisted of 0.2 mL of egg albumin (from fresh hen’s egg), 2.8 mL of phosphate-buffered saline (PBS, pH 6.4) and 2 mL of varying concentrations of diclofenac so that final concentrations become 31.25, 62.5, 125, 250, 500, 1 000 毺g/mL or the sample. A similar volume of double-distilled water served as control.

Is there any way to obtain a higher absorbance?

Thanks in advance