Hello,
I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine more efficient. My question is, if I remove this sequence, would it affect the stable transfection of my fibroblasts?
What is the function of this AAV2 ITR sequence in vectors?
Thank you very much in advance!
Hi Erwin, ITR is just functional when packaging AAV. If you just want to express a gene in cells, ITR would have no effect, no need to delete it.
You could find more information about AAV on this website: www.genemedi.net/i/aav-packaging
You need the AAV2 ITRs if you want to use the plasmid to generate recombinant Adeno-associated virus (AAV). These Inverted Terminal Repeats (ITRs) are palnidromic DNA sequences that form hairpin structures that are necessary fror AAV replication and encapsidation of the DNA into viral particles. If you don't want to use the plasmid to generate recombinant AAVs, you don't need the ITRs. They don't have any function if you just use your plasmid for transfection to express some transgene.
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