Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How can we make other Machine Learning Algorithms Deep like Deep Neural Networks?

For example Decision Tree, Ant Colony Optimization etc.

26 December 2020 6,804 3 View

Please help me with this question?

In a heat exchanger, the cold stream is a corrosive material and it flows through the tube side. The shell side fluid is at a higher pressure than the tube side_ Recently one of the tubes in the...

01 December 2020 2,860 6 View

How adsorbed Mercury (II) react with HCl and HNO3 in water?

Case: Hg2+ is adsorbed on a titanium carbide and 0.1 M HCl/HNO3 is used for the desorption of Hg2+. During the reaction, the solution turns into a pale yellow color. I want to understand what...

16 November 2020 5,770 3 View

Help for data analysis?

I am working with raster data for ANN, i have converted rasters to numaric and then test train that numerical data and get very Good accuracy. now i need to convert my train data into raster using...

14 November 2020 9,902 2 View

How to link excel with Aspen plus?

Is there any way to connect excel data sheets with aspen plus as inputs to perform parametric analysis?? I want to change input parameters for a certain range to see system performance

28 October 2020 2,174 3 View

Help me about swelling behavior of graphene oxide nanocomposites with methyl methacrylate with different proportions of GO?

Swelling behaviour of PMMA with GO contents 0.1%, 0.2,0.3,0.5?

02 October 2020 2,655 3 View

Please share your ideas and research for future PhD candidates in Meteorology research direction?

Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Meteorology Direction related to Hydro Meteorology, remote...

22 June 2020 3,475 16 View

Share your ideas for future PhD candidates in Environmenal Sciences research direction?

Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Environmenal Research Direction related to remote sensing,...

21 June 2020 1,539 28 View

Why I am observing a voltage drop from 2.4 to O V while discharging Lithium Sulfur battery?

I made a Lithium sulfur cell, with Sulfur/Carbon cathode and Lithium metal anode using 100 uL of electrolyte (0.6 M LiNO3 and 0.4 M LiTFSI in DME:DOL). I am using a Biologic VSP 300 battery cycler...

27 May 2020 1,232 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View