I am trying to detect Bin1 in DG75 and IIA1.6 cells. However the several antibodies that I tested all gave different band patterns. Does anyone have any experience with monoclonal/polyclonal antibodies against Bin1 ?
(1) Talk to the Ab supplier to get their Ab validation data;
(2) If possible, include positive and negative control cells;
(3) If possible, try other technology with the Abs, such as FACS.
in a human setting I would propose: Anti-BIN1 antibody [EPR13463] (Abcam)
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