Arsh Arsh

5 Questions 12 Answers 0 Followers

Questions related from Arsh Arsh

Protein not knocked out after CRISPR/Cas ?

I performed CRISPR/Cas to knock out an adaptor protein from two established cell lines (mouse and human). I performed multiple rounds of sequencing of the segment of target exon where the guide...

04 April 2019 8,205 14 View

Which is a good antibody to detect Bin1 or amphiphysin II in B cells via Western blotting ??

I am trying to detect Bin1 in DG75 and IIA1.6 cells. However the several antibodies that I tested all gave different band patterns. Does anyone have any experience with monoclonal/polyclonal...

29 March 2019 7,579 2 View

Protein detected by Mass spec but not on western blot ?

I performed affinity pull down using peptides and subsequently performed Mass Spec analysis. A protein that showed significant interaction with the peptides in my Mass spec analysis does not show...

03 March 2019 2,180 8 View

Which is a good antibody to detect Bin1 or amphiphysin II in B cells via Western blotting ??

I am trying to detect Bin1 in DG75 and IIA1.6 cells. However the several antibodies that I tested all gave different band patterns. Does anyone have any experience with monoclonal/polyclonal...

03 March 2019 1,172 2 View

Ponceau works but no bands seen on the western blot ?

I am trying to detect PIkBalpha on my blot. The Primary and secondary antibody dilutions prepared were absolutely fresh. O/N incubation with primary antibody after Ihr RT blocking and 1 hr...

01 January 2019 4,970 10 View