I am looking for some suggestions for internal standards that could be used in the fermentation process (using streptomyces). I have not found a single use of an internal standard in this matter, so any suggestions and ideas are helpful.
I would like to put this internal standard directly into the fermentation broth or the feeding solution at the beginning of the process without disturbing the process itself. For that, probably no intake of the standard should happen, it should not disturb the microorganism growth, also the standard should be non volatile and preferably detectable by LC-UV.
The use of this standard would be to control the fermentation broth evaporation (small scale), sampling and everything after the sampling (sample preparation).
To choose your internal standard it is important to know what kind of compounds you are going to measure as well as the the analytical method. The compound selected to be used must be compatible with your sample, not present in your sample and from the same nature as the compounds you want to evaluate.
Check https://link.springer.com/article/10.1007/s12161-021-01991-9
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Hi Nina! I am looking for exactly the same thing. Did you have any luck in finding a good internal standard?
Hi Anna Burniol-Figols , we are still searching for options. We used caffeine in the past since it did not seem to affect our microorganism we were working with at that time.
Now we are preparing to screen multiple options to figure out what could be used. Maybe a sugar source our microorganism does not have the ability to utilize.
If you find any good options, it would be greatly appreciated if you could share.
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