How to get consensus of results produced by four miRNA target prediction algorithms (RNA22, miRanda, TargetFinder and psRNATarget)?

Different miRNA target prediction algorithms use different scores which is not comparable directly. So what is the most reliable way to get the consensus of results produced by different miRNA...

24 August 2020 9,659 4 View

Is it useful to have consensus of results produced by four miRNA target prediction algorithms (RNA22, miRanda, TargetFinder and psRNATarget)?

RNA22, miRanda, TargetFinder, and psRNATarget are four different algorithms aiming at finding miRNA targets. Many research-work claims at using a consensus of the result produced by these...

23 August 2020 5,058 5 View

How we can calculate 17O hyperfine coupling constant in gaussian?how we can write the input file?

How we can calculate 17O hyperfine coupling constant in gaussian? how we can write the input file?

16 January 2020 5,197 3 View

How we can calculate the pH effect in gaussian?

How we can calculate the pH effect in gaussian?

04 October 2019 4,819 3 View

Can propidium iodide (PI) stain live cells also? I mean can it pass through the membrane via transporters or live cells with leaky membranes?

In order to confirm viability of cells, I used vital dyes like FDA, resazurin. I got positive for that at the same time cells have shown positive for PI as well. If my cells are viable, can they...

10 January 2019 658 2 View

What will be the probable aspects related to dairy farming(both organised and unorganised sector)?

Dairy development and allied activities

22 March 2017 6,211 3 View

In user level thread, when one high level thread preempt low level thread then how CPU context is switched without the involvement of kernel?

in ULT, scheduling is performed at user level (without the kernel involvement). but if one user level thread leave CPU and another will be schedule, then we have to change the value of program...

15 August 2016 8,551 2 View

How to perform centrifugation of silver nanoparticle solution to remove nitrate ions?

the precursor used was silver nitrate

23 May 2016 7,592 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

No title

22 February 2021 9,932 11 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View