Kindly suggest some institutes and contacts.
The Karlsruhe Institute of Technology
I am trying to use the two-dimensional Gaussian method mentioned in these research papers (Understanding the spatiotemporal pattern of the urban heat i... ; URBAN HEAT ISLAND FOOTPRINT EFFECTS ON...
24 January 2024 8,604 0 View
I am inducing callus from an aromatic Indica rice.
08 December 2023 8,235 0 View
suppose: we have a purified protein with us; after this step what are the methods and procedures that can we use to see the protein is intact- KINDLY HELP
01 June 2023 3,545 3 View
can there be results where a colony PCR showed a positive result to a gene cloned in a vector ; but after restriction digestion of that same recombinant clone; there is no band of that particular...
17 March 2023 3,141 4 View
I am trying to optimize certain primers; but only primer dimers are seen after trying with a lot of temperature range. What are the troubleshooting that can be done other than changing the...
16 January 2023 6,231 2 View
Hello,I want to change the wettability of the glass beads, from hydrophilic to hydrophobic. Will Silanization process (Tri Chloro Methyl Silane) work for it? Will it permanently change the...
20 January 2021 5,282 9 View
While doing NBO calculation for the lanthanide complexes in Gaussian I encountered this error several time "Error termination via Lnk1e in /home/apps/iitg/g16/l607.exe". Can some one please help...
01 January 2021 6,194 1 View
Different miRNA target prediction algorithms use different scores which is not comparable directly. So what is the most reliable way to get the consensus of results produced by different miRNA...
24 August 2020 9,934 4 View
RNA22, miRanda, TargetFinder, and psRNATarget are four different algorithms aiming at finding miRNA targets. Many research-work claims at using a consensus of the result produced by these...
23 August 2020 5,360 5 View
How we can calculate 17O hyperfine coupling constant in gaussian? how we can write the input file?
16 January 2020 5,352 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
