Is PAMPA an effective option? what membranes can be used to evaluate the same? are there options available in cell culture using SHSY5Y cell lines?
Any comments are appreciated!
Thank you.
Hi Niyamat Chimthanawala
Human brain microvascular endothelial cell lines (HBMECs) could be used to assess BBB permeability incase you do not have a trans-well system or microfluidic BBB models. SHSY5Y are not directly suitable for assessing blood-brain barrier (BBB) permeability.
Thanks,
Unfortunately, none of the cultured human brain EC models are tight enough to simulate in vivo flux of a small molecule. Use the permeability coefficient from one of the standard cell (MDCK, Caco-2) monolayer models. It is also important to determine whether the compound is a P-glycoprotein substrate and how bound the compound is in plasma. Ideally, measuring a 'calculated free' brain conc. after oral dosing is the best guesstimate.
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