Which housekeeping genes would you recommend for qPCR in dermal fibroblast and hepatocytes?

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Zonghan Gan

GAPDH

Susan C Hubchak

That's a good question. Best to use 2-3 target housekeeping genes to see which one(s) do not change under your experimental conditions. Some candidates would be GAPDH, 18S, TBP, HPRT1, beta-2-microglobulin. Good luck!

Jitendra Kumar Shandilya

Dear @A.K. Singh, for hepatocytes, GAPDH would work fine but for dermal fibroblasts select your reference gene wisely.

Hope the attached file have better information. They have run 22 genes in different condition, and found few stable reference gene.