Open Access Journal Research Center, a predatory publisher?

Dear Peers, I recently received an invitation as a guest editor from a journal under Open Access Journal Research Center (OAJRC). The OAJRC is not listed in the predatory publishers, but the...

09 November 2020 1,575 7 View

How to avoid particle aggregation when using Laser Direct Infrared (LDIR) to analyze microplastics?

I am trying to use LDIR system to identify microplastics of different types. However, significant aggregation of microplastics was observed during analysis. Especially for particles with a...

17 September 2020 1,532 1 View

Are there any platform to publish analysis protocol for human factors assessment?

I only find a BMC journal "Trials" to publish " randomized controlled trials in health ". https://trialsjournal.biomedcentral.com/ Are there any platform to publish general protocol for...

02 July 2020 4,691 3 View

DNA absorbance test on SpectraMax Microplate Reader, why A260/A280 false?

Hi guys. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime,...

29 March 2020 5,972 3 View

How can I observe my bacterial pellet after a centrifugation of a low concentration suspension ?

Hello, I'm currently working on DNA extraction from E. coli suspended in PBS. I'm working with concentrations ranging from 102 CFU/mL to 105 CFU/mL (and 108 CFU/mL as a positive control) and I...

02 March 2020 7,522 14 View

What progresses have been made in improving the assessment of motivation for a career with high income, high pressure, high risk and high expertise?

Hi all, For example (just an example), it will be a huge waste of time and money if a Ph.D candidate in physics at MIT becomes bored with academia or is scared away and withdraws. Then how can we...

07 November 2019 815 3 View

Control chart for kinematic viscosity?

I am interested to hear from people's opinion from the oil industry, or any company who performs QC check with kinematic viscosity. We got a CANNON kinematic viscometer in lab. It seems to be a...

27 October 2019 8,369 3 View

How can i implement Thomas-middlecoff method in matlab?

Hello everyone, I wonder if anyone can give me some advise on the matlab code to generate a mesh by solving the elliptic equation. Its some basic knowledge in the field of CFD, with which i am...

18 December 2018 6,181 1 View

How do I compare two time series volatility is similar or not?

11 December 2018 9,566 2 View

Can anyone offer me a booklist about learning algebraic geometry?

such as the book written by Hartshorn

07 September 2018 8,968 11 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View