How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

What are the mechanism of actions of Ag and Cu nanoparticles on the bacteria and fungi?

We are assessing the activity of nanoparticle incorporated fabrics against bacteria and fungi.

24 November 2020 8,686 6 View

How to estimate nitrogen content (%) in plant using pelican equipment's KELPLUS ELITE-EX VA semi automatic kjeldahl distillation unit? ?

I am using KELPLUS ELITE-EX VA semi-automatic Kjeldahl distillation unit for nitrogen estimation. The salesman told me that unlike the older version; this version does not require making up the...

01 October 2020 844 3 View

Why Magnesium Sulfate is used in Loop-Mediated Isothermal Amplification(LAMP) reaction instead of MgCl2?

We have designed an in house LAMP assay for the detection of a pathogen. We have used ThermoPol buffer, betaine, MgSO4, Bst polymerase and dNTPs in the reaction. I wanted to know why MgSO4 is used...

22 September 2020 6,916 5 View

Cloning in TRV2 vector: tips?

So, I have been cloning a lot of genes over the years in various plant transformation and overexpression vectors and even though its a struggle always to generate positive clones, I usually am...

26 August 2020 8,364 9 View

Is it possible to hire a portable photosynthesis system in Northern India?

I have an experiment in which I want to estimate the photosynthetic activity and carbon dioxide flux of wheat plant. However, in my institute does not have potable photosynthesis equipment. Is...

11 August 2020 9,701 2 View

Is it possible to publish research paper on plant nutrition having only single year of research work in a Thompson and Reuters IF Journal?

As in Masters of Science in Agronomy in many places have only one year of research work, is it possible to convert it to a paper and publish it in an impact factored journal especially when the...

04 August 2020 4,252 6 View

Has anyone used CABS flex 2.0 for molecular dynamics simulation of docked protein-protein structures?

Hi all, I need an info on the CABS flex and how it works for protein protein interaction simulations. I have docked structures obtained using cluspro and would like to subject them to MD. Please...

24 April 2020 1,509 1 View

What is the difference between Bubble type and Vapor type Precursor?

I am working with La(PrCp)3 Precursor in my Lab. I am not getting enough thickness after oxide layer deposition with ALD. To solve this issue I want to know about the precursor's chemical...

16 February 2020 5,640 3 View

How to register an indigenous variety in India?

The farmers are cultivating an unregistered land-race for a long period; if I wish to register this variety, what are the legal procedure and how to register it.

14 February 2020 525 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View