Which fluorescent markers I can use to stain live dead cells treated with doxorubicin?

23 May 2019 1 8K Report

Hi,

Which is the best live, dead flourescent stain to use if I have to image a spheroid treated with Doxorubicin. The standard live /dead assay kit has Calcien-AM (live) and ethidium bromide (dead) markers, but since Calcien-AM and Doxorubicin has overlapping flourescnce sepctrum, I can't use this kit. Any suggestions which markers I can use to stain live dead cells treated with doxorubicin?

Agnishwar Girigoswami

You can try with Calcein AM / Ethidium homodimer.

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