Is there any information about goblet cell physiology and morphology in rodents?

Rodents goblet cells.

05 June 2019 6,509 1 View

I am looking for a publication about reproductive system's micro- and macro anatomy in guinea pig. Can somebody help me?

Micro- and macro anatomy of reproductive system in guinea pig.

05 June 2019 2,405 3 View

Is there any findings about histological properties of sex glands in male rodents?

I want to do a research project about rodents sex glands histology. So, anybody knows new findings/articles in this regard?

10 November 2018 7,023 1 View

Is there any evolutionary reason or new insight about absence of gallbladder in some vertebrates?

Absence of gallbladder in some vertebrates...

08 September 2018 7,006 3 View

What are the similarities and differences of spinal cord histology between lower vertebrates (for example fish) and higher ones?

Spinal cord histology

08 September 2018 5,900 1 View

For fixation and staining of fish skeleton, which method is suitable?

Fish skeleton fixation and staining

07 August 2018 3,194 0 View

Does anyone knows the best method of fixation and staining of the teleosts bone?

Fish bone staining

04 May 2018 5,800 0 View

What is the apropriate method of osteoblasts counting in tissue slides?

Osteoblasts counting

02 March 2018 4,429 0 View

Absence of some organs in vertebrates

Why some organs are absent in vertebrates? for example rat lacks gallbladder. Is there any evolutionary or physiological reason?

01 January 1970 4,528 8 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View