Dear Raja

Many parameter can play a role in fertility.

Your question is general.

For example

1- Broodstock nutrition at pre-spawning

2- Sex steroid levels

3- Thyroid hormone

4- Ovarian fluid

5- Sperm quality and quantity

and.....

If you need further assist do not hesitate and message to me.

Here is my E-mail

[email protected]

Kind regards

H.A

Standardization of protocol plus supply of additives (energy rich compounds).

I am working on human and animals but I believe all paramertes of hematological of animal and others biological samples have standard units amange them .

also that associated with semen of their , we're the reson about any biological specimens apply the same principle.

selected broodstocks,good feed with required nutrition factors, suitable culture photothermal condition, and high-efficient management strategy.

Heat ,light and reroductive hormones activity

Hi..

Many reasons such as

1- Sex steroid range

2_Thyroid hormone

3- Ovarian fluid

4- Sperm quality and quantity

5- feeding and health of testicular

Thanks