Which extraction/purification kit would you suggest in isolating high-purity tRNA from archaea?

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Laurence Prunetti

You know that at the opposite side of the micro dept Valerie works on tRNA and the lab did extraction from Haloferax. just saying!

Artemis Louyakis

Hi Nat,

I've had a lot of luck with the MoBio kits. For my thrombolites, I use the biofilm kit and it has given me the most purified RNA of any of the methods or kits I used previously. Is there a particular contaminant that you are trying to remove? Or working with low cell counts?

Artemis