E. coli should be able to handle that, it is a bacterial protein initially after all. I would try E. coli first before going to the more complicated systems. 

Hi

check your protein solubility in E coli  at  http://biotech.ou.edu/   .

according of the web-tool, if chance of over-expression is below 50%; use a solubility enhancer tag (MBP,Trx,etc).

to choose between insect cells and yeast as alternative expression systems, i would rather to use yeast (Pichia pastoris ) .

for more information check below links :

http://biotech.ou.edu/

http://link.springer.com/protocol/10.1007%2F978-1-4939-2205-5_23

http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-4939-2205-5

Thanks for the answers, I am going to give it a go with coli and see if it is happy or not.

:-)

If it doesn't work, Mohammeds answer is very good. We were using both yeast and insect cells to express the dynein heavy chain (~350 kDa) in the Carter lab once upon a time. They each have their pros and cons of course. 

Frenchie,

I think if it is a bacterial protein it is best to handle it in e-coil system:-)