3 Questions 6 Answers 0 Followers
Questions related from Thierry Izoré
Hi, I plan to overexpress a very large bacterial protein (350 kDa). I have some doubt about how e.coli can handle that. Anyone to share some advices? Is it then better to try insect cells or yeast...
22 March 2017 547 5 View
Hi, I would like to know if anyone has tried DNA recombination in P. aerophilum. I would like to KO a gene but I don't know if tools are available for this organism. Many thanks.
12 August 2014 3,639 2 View
I am working on an actin-like protein which is supposed to form filaments. However, I noticed that the filament formation in my case was very poor, whatever the buffer I used (usually, 50mM Tris...
19 July 2013 9,226 6 View
