I would like to observe microgel particles inside fibres using UV microscope, thus I came acrross some difuculties when choosing the appropriate UV dye. The microscop can emit light at 405 nm, 488 nm, 555 nm and 633 nm. The dye has to be soluble in water, as other solvents like methanol and DMSO damage the fibres.
Karen A. Darbinyan
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Patrick Heinrich
Hi,
I would recommend to simply go for epifluorescence imaging using fluorescein, which is well soluable in water and perfectly excitable at around 488 nm. Also, an adequate standard filterset is usually available on most microscope setups.
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