How to extract peak coordinates from a given cluster with MATLAB as in the SPM results viewer?

Hello everyone, I am trying to extract peak coordinates from given clusters in an 3D fMRI group image, e.g. a thresholded ICA z-score map. Using MATLAB/SPM I am able to extract a single peak...

26 January 2021 7,672 2 View

I need to do a qRT PCR but there are not available filter tips?

Due to the COVID situation it is nearly impossible to find filter tips. Is it possible to do it without filter tips by making sure to clean the pipette with RNAse zap before use and working...

17 January 2021 8,839 3 View

Should I move forward on a study if I find there is a weak correlation, but not statistically significant?

I'm conducting a correlational study (Spearman rho) and there are no statistically significant relationships between the variables with a sample size of 37 (4 independent variables/1 dependent...

04 January 2021 5,976 5 View

Is there any index number for first author in journal article like h index?

In every research paper, first author contribution is comparatively high. To tribute their work is there any index number for an author acting as first author in different papers?

15 December 2020 8,231 14 View

Time to prepare samples for RNA-seq?

Hello, I am analysing the cost benefit of doing: RNA extraction and library preparations myself Sending RNA samples for sequencing Sending the entire sample for extraction and RNA preparation...

26 November 2020 2,203 2 View

How to identify original journal website?

There are two websites for few journal with same journal title and same ISSN number. For example, There are two websites for the journal titled "High technology letters (ISSN: 1006-6748...

18 November 2020 7,650 6 View

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

07 October 2020 739 3 View

Appropriate stats test for multiple DV, multiple treatments, paired across days?

Hi all, I'm trying to determine the right stats test for the project I'm working on. The setup is: 3 different treatment groups e.g. A, B, and C The same treatment is applied across different...

03 July 2020 4,487 7 View

Should there be sex education in schools and institutes about female menstruation?

02 July 2020 5,724 13 View

Do you have a specific question in glaucoma that may need a systematic review and/or a meta-analysis?

I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this...

21 May 2020 6,692 1 View

Does anyone grow Mycobacteria in TECAN Spark machine to get growth curves? What setting is necessary? Any tips (use Tween20 or tyloxapol?)?

I would like to grow Mycobact. Smegmatis in 24well plate in TECAN Spark machine. So far, I was using 100ml erlenmeyer flasks with 20 ml bacterial culture w/ shaking 180 rpm in 37C . Tecan would...

10 January 2021 6,624 2 View

Is there a possibility that nontuberculous mycobacteria (NTM) can be isolated in public playgrounds?

Since the nontuberculous mycobacteria (NTM) are environmental pathogens in which they are found in water, soil, and dust, is there a possibility that NTMs can be isolated in public places or...

06 October 2020 4,878 6 View

What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...

03 May 2020 1,332 2 View

KEGG pathway mapping of genes and proteins in prokaryotes?

Hi, I have a list of differentially expressed genes and proteins in mycobacteria and I would like to map them to KEGG pathways - could anyone advise how to go about it?

21 March 2020 1,308 3 View

M. tuberculosis is very slow growing, can I still plate it for a disk diffusion assay as I did on the fast growing Mycobacteria sp I used to screen?

I used a non-pathogenic fast growing Mycobacteria to screen my natural product compounds for activity. Now I want to test the active compounds in a disk diffusion assay against pathogenic...

01 February 2020 7,848 3 View

Choice of stain for visualization under Confocal microscope for mycobacteria?

Dear All, I have a couple of GFP tagged proteins that I want to study for localization in Mycobacteria. Which stains should be utilized for this purpose (if the cell wall to be stained). Can...

31 December 2019 4,708 2 View

What is a good nutrient agar for plating Mycobacteria tuberculosis for a disk diffusion test and why can't Mueller Hinton agar be used?

After I did some disc diffusion assays with a fast growing Mycobacteria I now want to do this using M. tuberculosis but I read that you cannot use Mueller Hinton agar with this. Does anyone know...

11 December 2019 7,868 3 View

What are the softwares that can be used to reduce the number of contigs in a whole genome sequence?

If there is any online software that can reduce the number of contigs or forming longer sequence in whole genome sequencing for mycobacteria species? Thank you very much!

05 June 2019 252 1 View

How do you bind lipids to an ELISA plate?

I am trying to bind lipid extractions from mycobacteria to Nunc PolySorp 96 well plates to perform ELISA's. Lipids are insoluble in water, so when I try and dilute down my lipid fractions (in 2:1...

03 April 2019 6,531 3 View

Hi everyone! Does anyone has experience in RNA extraction from mycobacterial cells grown as biofilms (both bead beater based or any other method)?

I am interested in studying the gene expression using Real time PCR and looking for a method that works for isolating RNA from mycobacterium marinum biofilms. Kindly share the protocols Thanks in...

01 February 2019 7,428 9 View