Is it possible to use natural dyes with comparable properties to existing gram/acid-fast stains as alternative?

24 February 2023 1 9K Report

Given that gram and acid-fast staining employ a number of dyes and a decolorizer, is it possible to substitute natural dyes with comparable properties? In acid-fast staining, mycobacteria, for example, contain long-chain fatty mycolic acids. Is it possible to substitute naturally occurring plant-based dyes with lipid-soluble properties?

Chathura Abeywickrama

Hi Jebb Consigo In general Styryl type (pyridinium) dyes exhibit good bacterial membrane staining ability. if you can find natural analogues, it is worth trying. Good Luck!

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