Which drug can I use to create microparticles of dextran?

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Problems with Sybr green melting curve?

Hello, Do you have experience with this type of melting curve? Can anyone explain to me why there is a lowering of the curve? I'm using SYbr select master mix on quant studio 5 real time pcr.

21 May 2024 1,191 0 View

Noves Ricerca Group?

Good morning to all, I have received an invitation to publish a paper from Noves Ricerca Group in a Q2 Journal. They have read my paper and very quickly they have accepted it for publication. It...

20 May 2024 7,407 3 View

What type of high-quality nanopure water do you use for NTA analyses?

We recently acquired a ViewSizer3000 from Horiba, and the manufacturer recommended us to use nanopure water from XZero for the NTA analyses. Do you recommend using any other brands of NW? Which...

05 May 2024 1,976 1 View

What is the best way to concentrate the protein and remove the imidazole post Ni-NTA purification?

I've always struggled with concentrating and washing my proteins after purification. Often I lose almost everything in the process. I have used dialysis membranes followed by column filter...

16 April 2024 5,716 0 View

Orbitrap Astral Mass Spectrometer: applications?

Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...

10 April 2024 9,346 0 View

Has anyone experience with a good one antibody for the western blot detection of SQSTM1 in human cells?

The old antibody has been discontinued and the currently offered are detecting a band at 62 kDa, which does not reflect the correct molecular weight (47 kDa).

17 March 2024 4,300 2 View

What can be the causes of which protein bands don't run through a SDS-PAGE gel?

I'm having a problem with SDS-gel. I cannot get the protein bands to run fully on the gel. For me it's the first time with SDS-gel. I prepared a polyacrylamide gel with 5% stackyng gel and 15%...

10 March 2024 228 2 View

Seeking Reviews for Q-TOF LCMS-9030 Mass Spectrometer?

Dear Forum Members, I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing...

10 March 2024 9,081 0 View

Can I use this buffer made 1 years ago and storage a 4ºC?

The buffer contains: 100mM NaCI, 0.1% Triton X-100, 300mM Sucrose, 1mM MgC2, 1mM EGTA, 10mM PIPES (pH 6.8), and 100uM PMSF

23 January 2024 4,464 1 View

Gerrymandering or how to detect discriminative electoral rule change? it?

In public life, democratic mechanisms can be misused. Fitting electoral rules to desired results is called Gerrymandering to remember a prominent politician who was an expertin such Machiavellian...

03 January 2024 2,849 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Does anyone know where to find polypropylene microspheres or microparticles around 1-5micrometer?

These will be used for the study of microplastics in the lung

29 July 2024 9,609 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View