I have been reading a lot about PCR and still I am ending confused. I need to know the difference between RT-PCR, real-time PCR, qPCR, RT-qPCR ?

I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...

11 February 2021 6,852 4 View

What are the new researches that are to be done in solar photo voltaic and solar drier?

what are new ideas to be develop..

18 March 2019 744 3 View

For elastomers I am not able to operate it at low voltages it requires very high voltage(2000v) for operation.Can anyone explain why i am facing this?

Please go through the reference paper for details. Carbon black is used as electrode.

12 April 2017 1,297 2 View

Why inhibition in NF-KB reporter cell line is not happening ?

22 May 2016 5,148 3 View

Any suggestions on why in Western blotting I can not get the bands of Lrp1?

Western blotting :In first i could get the lrp1 both bands but now its very difficult to get the bands. instead i get non specific bands .for cross check i have done the western blotting of old...

26 April 2015 339 7 View

For wind power potential estimation,how can you convert daily wind data to hourly data?

I have NCEP data for past and GCM dataset for future in daily temporal resolution.I need to convert daily dataset to hourly data.The attached paper suggests a method but I am not sure whether it...

08 February 2014 1,692 3 View

Can anyone brief me the dynamics between global climate models?

Lots of content is available online. However, its far too complex. I just want to know superficially the working of global climate models!

27 January 2014 7,979 1 View

No title

I am trying to solve the problem of linear dynamic instability by considering 2nd order approximation.

01 January 1970 6,968 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View