Which control should be used for my calculation: a positive which has standard compound or a negative control which has reagents only

More Disha Nayak's questions See All

Can any one suggest me alternate substrate for alpha amylase (PPA) assay?

For the plant extract antidiabetic activity assay I found Starch azure used as a substrate in a research article, can you suggest me any alternate substrate name because its time taking process to...

02 March 2017 4,420 4 View

Is it possible to dope iron (ii, iii) and Iron (III) nanoparticle with plant extracts?

I would like my active plant extracts doping on commercially available iron nanoparticle?? Is it possible?? If its possible than suggest me technique and reference. For supporting material suggest...

09 October 2015 4,252 1 View

Where I can analysis soil microbial community from any institute?

For Partial Microbial community analysis I prefer DGGE technique, suggest me any best technique to identify and if DGGE is available having minimum charges let me know the detail to avail the...

09 October 2015 4,333 0 View

Can we consider negative result of sample in antioxidant assay?

In all antioxidant assay for calculation below mentioned formula is used % of Inhibition = CONTROL O.D - Sample O.D/ CONTROL O.D X 100 But, In this case I got negative result of sample because...

07 August 2015 4,158 13 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Are current regulations effective in preventing cancer caused by toxins?

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02 August 2024 4,474 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

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01 August 2024 8,754 2 View

How to make a Lateral flow control line more uniform?

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31 July 2024 4,510 3 View

How do we pick data for determination of Validation Acceptance Criteria?

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23 July 2024 7,292 3 View

How I can add anew research in my account ?

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21 July 2024 7,545 0 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Ke-Wei Zhao

Disha: You have to be mindful that all your numbers are net readings (e.g., OD of control minus OD of reagent only, OD of sample minus OD of reagent only, etc). Then your equation should be fine.

Rajesh K P

At is of control you have to put the respective od value what you get for the reaction mixture without any test sample. I mean Zero concentration tube