Which concentration to use for XTT assay?

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Samir Ranjan Panda

Hi Aya Ali

The working concentration of XTT is 0.3 mg/ml for 4-6hrs. The detailed protocol of measuring cell viability using XTT has been attached below.

* https://www.sigmaaldrich.com/IN/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/cell-proliferation-kit-xtt-assay

Thanks

Hope it helps

Zeinab Basiri

Hi Aye Ali

How do you use XTT to identify the ROS?