What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

For Rt PCR?

Is it necessary to run the Rt PCR product on agarose gel after completion?

29 December 2020 4,823 3 View

How to interpret MSWEP 3-hourly rainfall data 0.1 degree resolution?

I have downloaded the MSWEP sub-daily rainfall data (3-hourly data 0.1 degree resolution). I used MATLAB to convert it and obtained a table of dimensions 1800 x 3600 for a specific date. I am...

18 October 2020 4,205 4 View

Fan boundary condition using Ansys Fluent?

I am trying to model a jet fan being installed in a room. I want to study the flow of the fan coming out of the outlet (basically the development of streamlines). The walls of the room are...

12 October 2020 4,970 3 View

In Abaqus- How to optimize the shape of a particular region while keeping displacement at two points equal?

I am new to abaqus and I am trying to optimize a shape of a segment of a model (As a part of my master thesis). Please refer to the example model I have attached. I need to optimize the segment...

23 August 2020 5,828 9 View

Shall I submit the article simultaneously to another journal even after request to first journal to withdraw the manuscript???

I have submitted one of my review article on COVID 19 and ophthalmology in April through online submission but journal haven't overlooked it inspite of several request. Even i have sent request...

06 August 2020 1,802 3 View

How do you transfer files from Open Foam to Star CCM ?

Hi I am trying to find some information as how to link up OpenFoam and Star CCM, however I am not able to find adequate information. Below is the details of the project. 1- Objective: to...

05 June 2020 8,484 3 View

How to start jupyter notebook from a particular folder instead of default root folder?

I am using python 3.6.8 on a Windows 10 machine. We can only move to subdirectories from the default installation (root) folder in jupyter. I wanted to start the notebook from a particular...

08 March 2020 9,259 4 View

Is it OK to anneal GaN HEMT epitax after some AlGaN recess. what are its pros and cons?

HEMT FABRICATION.

24 February 2020 4,958 3 View

Best resources for Multivariate Kriging for deterministic simulation with Python ?

Hello, I am new to surrogate-based data analysis. In my model, Input space consists 6 variables and a single ouput is noted from each simulation. I have N such simulations. ( Sampling method is...

09 February 2020 7,511 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View