Which buffer should I use for resuspension and storage of yeast TK ?

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Olga N. Solovjeva

I precipitate the yeast TK with ammonium sulfate pH 7.6 and suspend the precipitate in 10 mM potassium phosphate buffer pH 7,6, with the TK concentration 20-30 mg/ml. Then freeze it in portions. Stays several years without loss of activity

Julien Henri

Thanks for your insight, Olga.