Which buffer should I use for resuspension and storage of yeast TK ?

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Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to remove a sapphire disk from LR white resin embedding ?

Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...

13 January 2021 5,223 3 View

What do you suggest me as a low-cost electronic monitoring of honeybees using 3-5mg RFID tags?

We would like to control the honeybees' ID at the entrance of a flight tunnel. We would like to detect a honeybee in a cube of 10x10x10 cm3 in front of the tunnel door while flying, and thus...

17 December 2020 3,464 2 View

What are the competencies to put forward in Higher Education to prepare students to evolve in a world where IA is ubiquituous?

15 December 2020 6,350 4 View

Is it possible to invert a PLSR model, i-e to predict X based on Y?

There are many tools to build a PLSR model to predict a response variable Y based on a multivariate predictive variable X (reflectance spectra for example). My question is the following: once we...

06 December 2020 3,096 11 View

Will Oil Prices Ever Recover back to $65/bl ?

Oil prices have waxed and waned following COVID-19, but supply pressures and compliance from OPEC member countries have provided a much-needed balance int he last decade. With the U.S. becoming...

09 October 2020 479 2 View

Have you ever included two last authors in the co-authorship of a publication?

Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...

21 September 2020 2,995 5 View

How does one find constants of the motion for a chaotic flow?

I'm reviewing a lot of papers where the authors take a 3-D autonomous chaotic system (think Lorenz) and add a fourth variable bidirectionally coupled to the other three and then report its unusual...

04 May 2020 3,785 33 View

Device abstraction for navigation in VR ?

Hello everyone The UMI3D Consortium's working group dedicated to embodiment is currently looking for a device agnostic way to manage navigation in Collaborative Virtual Environment (CVE). Are you...

08 April 2020 4,951 3 View

Redundancy analysis (RDA) order variables change result, why?

Hi everyone. I am in the process of making RDAs between faunistic and environmental tables. What I don't understand is that depending on the order in which I place my environmental variables...

29 March 2020 2,179 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View