How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View

Is it possible to calculate the energy stored (kWh) in a EV battery by knowing the cathode discharge potential and specific capacity properties?

For example, if I have the following data for a LiNi0,8Co0,15Al0,05O2 compound used in the cathode: - Specific capacity = 200 mAh/g - Average voltage = 3,7 V I have no information about the...

16 January 2021 3,038 2 View

PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

Can you suggest a sub-problem and possible objectives regarding to this thesis/research study?

03 November 2020 8,155 3 View

DNA disappears from gel, whats going on?

Recently I've been doing some PCRs and the primes used to work a month ago, but now that we want to repeat the experiment, they are not working anymore. I have noticed something in my gels, and it...

28 October 2020 4,427 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...

18 January 2021 5,151 1 View

Does use of hand sanitizer negatively impact the skin microbiome?

Hello, Most hand sanitizers contain 60-80% alcohol. Alcohol destroys organisms including bacteria, fungi, protists, and enveloped viruses by breaking down the plasma membrane. In recent...

16 January 2021 6,923 11 View