Laser TIRF 3 works with up to a total of six lasers, including up to four solid-state lasers. You can use its line selection and attenuation device to easily choose the excitation wavelength and intensities for single molecular imaging. With an optional, motorized slider, you can reproduce your illumination angle with precision every time.

Laser TIRF 3 is also completely safe — intelligent laser safety gives you maximum protection at all times.

Olá Alejandro,

because I presume your applications will be coming from a biololgy bachground, I suggest to include a 488 line which is commonly used to excite GFPs etc. 561 is a good choice for red labels. 

If you can elaborate a bit about the application it would help the advise.

Kind regards,

Jens

That depends on what fluorescence you watch. 488 line is common for most dyes. If you observe Trp fluorescence, you need a UV laser; if you observe IR, you might want a red excitation.

I agree with the Guys. You need to be more specific about the dyes you want to use. Still, 488 is by far one of the most common. Because of that you can buy it for a good price as well. I advice you to also think about the laser power and the way you plan to couple it to the microscope.

Thank you all for your answers! In order to clarify, this system will be placed in a centralized microscopy unit and we need to balance the price with the upcomming needs of users. The idea is to have a versitile system which can be useful to the largest number of users. In this sense, I was adviced to give preference to CFP and YFP (probably TagYFP),  thus would need a 458 nm and a 509 nm (¿through the argon 488?).  Of course the sales reps suggest we get 4 lasers...

Well, I must say, I agree with the sales reps on this point. As it is a centralized microscopy unit, there will be many users with different needs. I advice to go all commercial: microscope and TIRF unit. With a bit of negotiation you can get a good price. From my experience, major microscope manufacturers provide good TIRF systems.  

In principle you could install a supercontinuum Laser and use a Filter to discriminate the color. This would give you maximum flexibility. Although I don't know whether any company uses whitelight lasers in a commercial TIRF setup.

Best regards

Benjamin

Thank you all for your answers!! Could the 514 nm line of the 488 be used while making a transition from one fluofore to other? Or is it to dim? I think many users will rely on their "true and tested" constructs at first before switching to mCitrine.