How to get rid of the free GFP signal in an Atg8-GFP tagged yeast saccharomyces cerevisiae?

I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...

15 November 2020 8,556 3 View

Hi all! I need to activate CD8 T cells extracted from mice spleen in vitro, which option is the best? Concanavalin A? LPS? anti-CD3 and anti-CD8?

in vitro mice CD8 cell activation. These cells are extracted from B6 mice spleen by magnetic separation.

01 November 2020 3,276 2 View

Hi all! What vehicle do you recommend for IP injection in mice for a DMSO soluble drug?

I have 2 drugs (PTC-209 and GSK343) that are soluble in DMSO (I already made the stock solution, dissolved to a clear solution) and I have to inject them IP to mice for a 2 week treatment course....

17 September 2020 9,107 3 View

Why is Quantum-dot Cellular Automaton (QCA) technology fail to flourish? Specially, Semiconductor and Magnetic QCA for electronic applications?

In 1993, Lent et al. introduced one of the most promising and emerging computational paradigm known as Quantum-dot Cellular Automaton (QCA) computing. Now, it is almost 30 years have been passed,...

06 May 2020 4,955 7 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

In which reason EDTA used in injectables containing proton pomp inhibitors?

We know that EDTA is used by different reasons (chelating agent, support for antimicrobial effectiveness e.g) in pharmaceuticals. Is there any specific reason to prefer EDTA in injectables...

01 December 2019 5,166 3 View

How to get mean age of fluid for a closed system using FLUENT?

Hi everyone, I am working on simulation of stirred tank reactor operating at batch mode. So, there is no fluid inlet-outlet in the system. Can I calculate the local mean age of fluid by using...

18 November 2019 1,142 3 View

How is the length of any scientific document (report, thesis, essay, conference paper, journal article) calculated ?

I wish to know what aspects of these technical documents are included and excluded while making a word count. Specifically, say, a figure caption, references, tables, table footnotes, page...

24 June 2019 9,815 3 View

Hey friends, I have a problem with my primers, is there any way to do PCR with one weak primer?

I have ordered a pair of primers, forward primer is working well, I have checked it by gel, but revers primer has really weak band in gel, and PCR doesn't work. any suggestion?

18 November 2018 5,958 3 View

I would like to simulate a mesh network in NS2. How can I do it? Which 802.11 I need?

Thanks in advance for your replies.

15 February 2015 8,579 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View