I am trying to detect Caspase-3 activity in the M1000 Prion infected mouse brain. I was using Anti-Caspase-3 antibody ab90437 product. It was giving a lot of unspecific band between 36-17 kd.
We list a few other sources you may want to consider, like Invitrogen's (ThermoFisher) mAb 31A1067:
https://www.linscottsdirectory.com/antibodies?q=caspase-3-active-pro
Also, there are a couple of reviews you should look at on www.pAbmAbs.com
Good luck.
Thanks Russell.
The quality of antibody affect a lot to the WB result, you should try some high quality commercial antibody.
I fund some product for you to consider:
http://www.creative-diagnostics.com/Search.aspx?keys=caspase-3&cid=4
or if you don't care about the price, you could consider antibody from Abcam.
Hope it can help.
Thanks Yudong
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