I am currently planning to sample and screen streptomycetes from the soils of our university for antibacterial activity and would like to know which antibiotics would be an optimal point of comparison.
You could use anything that has antibacterial activity versus the strains you are using under the testing conditions, preferably a drug that is easily obtainable at low cost.
It depends on the organisms which you use for your assay.
For example, for Candida you can use commercially available Fluconazole, Pseudomonas- Gentamycin etc.
Depends on what kind activities you are looking for. Antibacterial, beta-lactamase inhibitor, anti fungal, or anti cancer...... There are many models.
Streptomyces are Gram +, so you have to add an anti-gram- like nalidixic acid and since the soil is full of mushrooms it is necessary to add in the isolation media or in the sample antifungals like nystatin, amphotericin B or the actidione. Good luck
Ampicillin against gram+ and gram - strain , and cyclohexemide as antifungials
Just nalidixic acid for Gram - and no anti-Gram + because streptomyces are also Gram +. For mushrooms add actidione.
Thank you for your kind responses! To clarify specifically, I hope to test them against Staphylococcus aureus, Streptococcus pneumoniae, and Salmonella enterica.
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