I am preparing the protein nanoparticles and trying to stabilize the nanoparticles by providing temperature. I have read in literatures that the amide bonds are formed between two amino acids in a protein nanoparticle stabilized by heat. I am using the 50 degree celsius temperature. I wanted to know whether the formation of amide bonds are effected by temperature or not. Like at 50 degree celsius the formation of amide bonds will be higher than at room temperature.
Are you considering the literature for peptide synthesizers for amino acids having tBoc and fMoc blocked side chains? Yes, temperature and pH have to be striclty controlled to get oligomerization and especially with those amino acids that have bulky side groups. So you can get more peptide bonds at 50C and better at 60C but also keep in mind that there may be greater side reactions.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Atomic, Molecular and Optical Physics
- Atoms