Dear All, I would like to publish a protocol on how to isolate purkinje cells from mouse brain on a scale that downstream analysis such as RNA sequencing could be performed. Any journal suggestiongs for this topic? Thanks.
We are seeking contributors to write on work-life balance in South Africa, Egypt, Tanzania, Indonesia, Argentina, Ecuador, and Columbia. Please signify your intention by emailing me @...
17 February 2019 1,414 10 View
I need help regarding a cell population that we have observed having PCNA expressed but not Mi67. How is that possible since both are proliferation markers. I can understand having Mi67 positive...
29 November 2018 8,080 1 View
To do some western blots and immunohistochemistry I am looking for a CAMTA1 antibody. We have tried already with Abcam and Santa cruz but they are not specific, to be precise they give signal in...
22 March 2017 4,738 2 View
I have RNA extraction from mouse kidneys. I had very good RIN values for that RNA extractions (around 9.0). Since I had bad experience with bacterial RNA contaminations I decided to measure the...
01 March 2017 1,041 5 View
Several of the most successful architects have been associated with maxims like Mies- less is more. Several have also been architectural theorists or teachers with schools of thought such as...
08 September 2013 4,572 1 View
I am studying the relationship between running a successful architectural firm and the practice ideology of the principal architect(s).
04 May 2013 2,515 0 View
This question is one of the research questions in a longitudinal study of knowledge, beliefs and practices of architects in Nigeria
04 May 2013 8,835 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View