Dear All, I would like to publish a protocol on how to isolate purkinje cells from mouse brain on a scale that downstream analysis such as RNA sequencing could be performed. Any journal suggestiongs for this topic? Thanks.
I have RNA extraction from mouse kidneys. I had very good RIN values for that RNA extractions (around 9.0). Since I had bad experience with bacterial RNA contaminations I decided to measure the...
01 March 2017 989 5 View
Several of the most successful architects have been associated with maxims like Mies- less is more. Several have also been architectural theorists or teachers with schools of thought such as...
08 September 2013 4,529 1 View
I am studying the relationship between running a successful architectural firm and the practice ideology of the principal architect(s).
04 May 2013 2,477 0 View
This question is one of the research questions in a longitudinal study of knowledge, beliefs and practices of architects in Nigeria
04 May 2013 8,806 1 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
28 February 2021 2,534 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View