Where is the Ribosome binding site in pOPIN vectors?

16 August 2018 0 1K Report

Dear all,

I have successfully cloned and expressed the recombinant proteins using pOPIN E and F vectors as backbone in E. coli. However, yesterday I realised that there is no ribosome binding site in the vectors. I have been looking up for any alternative way to recruit ribosomes in pOPIN vectors since. I have no luck so far. Can anyone shed some light on this matter?

Thanks.

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