Where I can find Mass spectroscopy for protein identification in Pakistan?

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What is the protocol for SDS protein lysis buffer?

Hi! I am trying to extract protein from brain samples and I want to use 2% SDS lysis buffer, I know the ingredients but I can't figure out the exact amount of those ingredients. Can someone help...

18 October 2023 7,463 3 View

How to perform lyophilization of protein gel bands?

I have performed gel electrophoresis and got the protein bands on gel but now I have to cut the bands from gel and perform lyophilization of these bands but I can't find the protocol for this....

23 September 2023 7,721 0 View

What can be a reason for an LC3B antibody to not work in western blot.?

I am trying to optimise concentration of chloroquine ()to assess inhibition of degradation of my protein) in cycloheximide chase assay for HeLa only and HeLa -B*38/39-HA. Unfortunately, i can't...

09 March 2022 9,291 2 View

Why many Universities require students to use APA (American Psychological Association) documentation style?

Why not give importance to other styles?

23 July 2020 288 5 View

Can we use both theoretical and conceptual framework for all kind of researches?

Or whether we use theoretical framework solely for quantitative research and conceptual framework solely use for qualitative research and use both framework for mix-method research?

10 April 2020 6,624 7 View

How do i extract pixel values of Chlorophyll a and Total Suspended Matter from the processed C2RCC images?

I am doing a research of monitoring algal blooms in inland water bodies How can i extract the pixel values of Chlorophyll a and Total Suspended Matter from the processed C2RCC images in SNAP. I...

23 February 2020 815 5 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View