How can I go about in silico studies using chemical compound identified in fraction from column chromatography using HPLC or GCM-M?

I want see if there is interaction with compounds characterised with either HPLC or GCM-M with any proteins in silico

31 December 2018 8,459 3 View

Can one remove slovent from nonpolar solvent extract through Lyophilization ?

lyophilizer can remove non polar solvent from extract just as with water

08 September 2018 7,029 2 View

Can i get a youtube link or any material on the use of graph pad prism ?

most statistical analysis or graph in my study are done using SPSS but i want to explore the use of graph pad prism.

04 May 2017 3,241 1 View

Can I get materials, jornal papers or links that can help in learning and mastering the use of PyMOL ?

I need the the latest application of the software application for the antimalarial principle identification in my study. I need more enlightenment on the usefulness in toxicological studies

04 May 2017 8,089 1 View

How can one synthesize ZnO naonoparticle using ZnO salt which is insoluble in water and aqueous laef extract?

The experiment is green synthesis of ZnO nanoparticle using aqueous extract.

08 September 2016 1,526 3 View

What is the ratio of plant extract to salt in green synthesis of nanomaterials?

this is to know the proportion of the extract that should be added to the salt to use for green synthesis

10 November 2015 3,504 4 View

How effective is calcination in nanomaterial green synthesis and which other methods could be preferred?

10 November 2015 9,259 7 View

Which statistical package should be preferred for more accurate result for biological experiments?

best statistical package for statistical analysis

06 July 2015 8,340 0 View

Can anyone send to me any publication or book on chemoinformatics and bioinformatic?

this to enhance my knowledge on this concepts

04 May 2015 5,114 0 View

Can one identify an unculturable microorganism using meta-genomic technique?

a fermentation of our local seed were fermented and we have some suspicious that certain other micro-organism could be responsible and want see if meta-genomic technique be useful in confirming it

04 May 2015 1,916 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View