Where can i learn plasmid construction step by step in detail?

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Why is there overgrowth on LB media after transformation?

I've been doing transformation several times with DH5. I got overgrowth/bacterial lawn growing on my plates twice, and when I transform for the 3rd time, there's no colonies formed. I'm using heat...

22 June 2024 4,843 1 View

Analysis method of ammonium bisulfite (as active substance of oxygen scavenger) ?

Good Day! Thank you for the opportunity, I would like to ask you. Is there any standard method (ASTM or API) for analysis of ammonium bisulfite (as active substance of oxygen scavenger) ? Thank...

29 January 2024 1,926 0 View

Hello, i am currently synthesizing molecularly imprinted polymer with curcumin as a template, which extraction methods can i use to remove a template?

Actually, i have already tried several extractions methods using 10% acetic acid slution in methanol, such as maceration, refluks, and sonication, but the template does not extract, so i hope...

17 January 2024 5,220 2 View

Why do my DH5a ecoli colony looking weird?

Usually, my ecoli colony has a distinct shape and doesn't form a hairy look like this. I use LBA with AMP 100 µL/mL. The plasmid inside my ecoli has the AmpR region so they should survive under...

10 January 2024 9,531 2 View

What is the theoretical reason behind the exact number of research participants for interview in qualitative descriptive?

i am currently doing a thesis of qualitative descriptive research about students computational thinking ability. based on other research that i read, they categorized the student's ability into 3...

04 December 2023 5,140 2 View

The most suitable diffusion coefficient for hidrogen in membrane reactor ?

currently I am modeling the membrane reactor. hydrogen (reaction product) as a permeated substance. when modeling a packed bed reactor I use: D=(U*Dp)/(11*(1+(19.4*((Dp/(d1*2))^2)))) D=...

06 September 2023 800 1 View

Seeking Guidance for Membrane Packed-Bed Reactor Simulation Research ?

I am currently engaged in the modeling of a membrane packed bed reactor, specifically in its initial stages where only a packed bed reactor is considered, and the model has not yet incorporated a...

02 July 2023 2,132 3 View

How do permeation effect on the velocity inside the reactor?

right now I'm modeling a membrane pack bed reactor. but I haven't been able to get the appropriate results because I can't connect the effect of the permeation that occurs to the velocity inside...

05 June 2023 2,558 1 View

Something brown passed through my Nafion-117 membrane. Is it normal?

Hello, I'm currently doing experiments on microbial fuel cell. I am using two interconnected chambers (anodic and cathodic) for my mfc, separated by a Nafion-117 membrane. As the catholyte, i...

22 May 2023 6,599 0 View

I'm doing research on the Black Litterman Portfolio. I have 489 stock return data with zero, negative and positive values. How I see the normality?

I'm doing research on the Black Litterman Portfolio. I have 489 stock return data with zero, negative and positive values. How can I see the normality of the data? I have tried the Kolmogorov...

30 April 2023 407 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View