Virania Putri

4 Questions 4 Answers 0 Followers

Questions related from Virania Putri

What protein purification method should i use for recombinant spidroin?

I'm going to express recombinant spidroin from a pET-22b(+) plasmid, the protein MW is around 51 kDa and I want to study where the protein is expressed (extracellular, intracellular...

02 February 2025 5,597 3 View

When is restriction sites needed in primer?

I need to design a pair of primers for colony PCR. Do I need to add a restriction site, or do I just need to add 5' upstream bases? And what delta G is tolerable for intra- and intermolecular...

21 November 2024 4,233 3 View

What type of primer design is best used on synthetic plasmid?

This primer will be used for colony PCR. My lecturer suggested that I choose a region that includes both the backbone and insert gene so that it can ensure specificity. The plasmid is pET-22b(+),...

12 November 2024 7,199 3 View

Where can i learn plasmid construction step by step in detail?

Hi everyone, i'm currently doing my thesis research and I need a suggestion for a learning supplies. For more context, my goal is to express a chimeric recombinant spidroin containing a...

20 July 2024 8,989 2 View