Does activated charcoal adsorb aflatoxins M1 in milk?

some studies have demonstrated in vitro that activated charcoal can adsorb aflatoxins. more citations would help me have evidence to the scientific...

14 November 2019 211 4 View

I was only able to generate forward sequences for some of my matK, rbcL and ITS1 genes and will they still be useful for my phylogenetic analysis?

I sent some plant DNA for DNA sequencing and apparently, though sequences were sent back, some pairs don't create a consensus sequence. The sequencing company tried to re-sequence, bu to no...

26 April 2019 2,664 4 View

When collecting qualitative data, what systems do people use to keep track of the different data collected?

I would like to know if people have concrete suggestions about or know of bibliography or on line resources that describe how to keep track of the different data collected. For example, if...

02 March 2018 5,892 9 View

Can someone please share a Matlab code to solve a system of (3) non-Linear ODEs (BVP) numerically using Runge Kutta and the shooting method?

30 April 2017 5,566 3 View

Does anyone have experience with growing Expi293F suspension cells in the spinner culture flasks?

I am currently establishing Expi293F cells in my lab using the spinner flasks and not the orbital shaker, but I'm having a few issues: I do not know the best rpm for growth, Cell viability is...

16 January 2017 7,526 1 View

Is there a trick to transfecting BV2 cells with siRNA?

I have tried several different reagents and monitored from 4 to 72 hours, I am not seeing toxicity, but I am not getting siRNA into the cell, no detectable GFP with contro and no knock down with...

16 July 2016 4,525 6 View

I have conducted a Moderation analysis with no interaction effect. Should I change analysis to Multiple Regression?

The moderation analysis was significant for both the IV and Moderating variables, but no significant interaction. This leads me to think I have chosen the wrong analysis and should continue with a...

03 June 2016 1,908 3 View

How to achieve a monolayer of 16hbe14o- cells on a transwell insert?

Hi. I am trying to culture 16hbe14o- cells (human bronchial epithelial cells) on 24/6 well transwell inserts. The cells grow well in T75 flasks but I am struggling to get a mono layer on the...

03 February 2016 6,707 1 View

How to achieve a monolayer of 16hbe14o- cells on a transwell insert?

Hi. I am trying to culture 16hbe14o- cells (human bronchial epithelial cells) on 24/6 well transwell inserts. The cells grow well in T75 flasks but I am struggling to get a mono layer on the...

01 February 2016 2,868 1 View

Can anyone help me solve my issue with thawing embyronic stem cells?

02 May 2015 283 9 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View