Where can I get a positive strain for E.coli O157 or its DNA?

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After an error questionairee is discarded what happens next?

If a sample size is 300 and 5 Questionnaires are discarded due to error, are the 5 discarded questionairee not meant to be replaced? Just curious

16 April 2024 7,472 3 View

Environmental Agency data Vs Citizen Science Data on water quality?

Are there discrepancies between the environmental agency data on water quality and data gotten from Citizen Science?

03 May 2023 1,334 3 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,565 5 View

Making vaccines using the virus's protein?

What is the process in using only parts of viruses such as proteins which can be recognized by the immune system to make vaccines such as in the case of the spike protein for Mers-CoV? I...

23 February 2020 1,297 5 View

What are the possible sources of error if i carried out Iodine staining on a TLC plate and no mark appeared?

I carried out thin layer chromatography and afterwards had to stain my plate with iodine as an alternative viewing method but the analytes did not take a brown or yellow color. The whole plate was...

20 February 2020 5,416 3 View

Comparing two chromatograms?

QUESTION I WAS ASKED: Compare the chromatograms in Appendix 1 and Appendix 2 and note your observations? MY QUESTION: What exactly am I meant to compare? I know you guys are not seeing the...

30 November 2019 3,711 2 View

What are the possible sources of error if I run a gel and can only see the DNA ladder under the UV light?

Hey guys, I did a restriction digest and ran my sample on an agarose gel. Under the UV light, all I can see are the bands of the ladder. A loading buffer was used.

29 November 2019 1,204 3 View

Is any one experienced in implementation research (IR)?

I am working on vaccines supply chain and I need assistance from someone who is experienced in IR. I am seeking for the best strategies to improve the supply chain and how to implement the...

16 February 2017 5,143 3 View

Do immunoglobulin A, G, M have any relevance in the "prevention from mother to child transmission of HIV" ?

Do immunoglobulin A, G, M have any relevance/importance/role in the "prevention from mother to child transmission of HIV"

06 July 2016 8,395 2 View

Can the immune system of HIV+ mothers determine if the child becomes HIV+ or not?

please i want an article that proved that the immune strength of HIV+ mother determines if the infant becomes HIV infected or not

05 July 2016 2,114 5 View

What can be yellow colonies after E. coli TOP10 trnasformation?

Last week, I transformed home-prepared E. coli TOP10 cells. When checking the colonies, I noticed that some of them have yellow color. They did not grow on the randomly, but dependently on the...

16 June 2024 3,272 3 View

Do DH5a and MC4100 competent cells Fluoresce?

Hello, I have currently been tasked with performing a split GFP analysis. I have been using DH5a cells for plasmid transformation. The results were turning out to be what we expected. The...

29 May 2024 5,791 0 View

What tool can predict E. coli protease cleavage sites?

I am observing cleavage in my construct expressed in E. coli and attempting to identify the responsible proteases. Mass spectrometry analysis has revealed the sizes of the fragments, enabling...

12 May 2024 6,540 2 View

Can you explain the difference observed between my PFGE and WGS results for E. coli comparison?

Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...

12 May 2024 8,107 2 View

Which agars should I use for the microbiological analysis of purified water ?

As a new microbiologist, I am currently working in a non-sterile production pharmaceutical factory where I take pure water samples from 13 points, fill 100 ml of pure water into 3 sample...

29 April 2024 7,096 1 View

How to predict the perfect bacteria enzyme for my newly synthesized compound?

Say I have synthesized a new compound that gives great results against e. coli, how to know the best enzyme to target in e. coli? or how to predict the most possible target?

18 April 2024 5,156 2 View

How can I reduce the variability of CFU counts between replicates?

I'm challenging bacteria with different compounds, then plating the suspension on LB agar to assess CFU and understand the bactericidal properties of my compounds. However, I'm encountering...

10 April 2024 7,459 7 View

How can I improve my plasmid DNA yield using CopyCutter EPI400 for a toxic gene?

I am trying to clone a large (11kb) and toxic gene in an expression vector. Therefore, I am using the EPI400 CopyCutter strain that is maintaining the plasmid at a low copy number to allow...

09 April 2024 2,861 3 View

How to confirm your ligation before its transformation in E.coli?

So let say I have circular plasmid named "PDR111". I did PCR with KOD ONE MIX KIT and the product is linear DNA. Then, I did self circularization of linear DNA with T4 DNA Ligase protocol (Thermo...

11 March 2024 1,143 7 View

Storage of overnight e. coli cultures?

need to do an overnight E .coli culture from a colony or grow it to at least OD of 1 but can't do the next few steps for overnight induction - what happens if i store the culture in the fridge for...

06 March 2024 5,497 2 View