What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

What are the parameters that plays a role in life-time degradation models of concrete? what are the most important tests to measure these parameters?

I'm working on a research about the prediction life-time models for concrete in harsh environment. I have to answer those two questions.

14 November 2020 2,043 7 View

How to get citations on RG to show up on Google Scholar and vice versa?

After connecting both platforms, there remains unique coverage; just this week I discovered a new citation of mine on Google Scholar, but it's not being recognized by RG?

30 October 2020 4,311 3 View

Does anyone know of historic sites that have artist in residency programs?

A research assistant and I are conducting primary research on artist in residency programs (artists, writers, etc.) and the use of contemporary art at historic sites to reinvigorate or...

27 September 2020 9,355 4 View

What is PROSPERO - University of York regarding Systematic Literature Reviews?

I have seen for the first time a systematic literature review and meta-analysis that has prospectively registered their review in PROSPERO and I am wondering what is the main reason for creating...

04 August 2020 4,167 4 View

What causes the copy detection limitation in PCR?

Hi all, I wish to understand why doesn't all polymerase can detect a single copy of region of interest? While some polymerase is more sensitive (like dreamtaq), and some are less sensitive, what...

02 June 2020 4,397 21 View

Evidence-based resources for helping kids cope with debilitating & likely fatal disease?

A colleague asked me for any evidence-based approaches to helping kids (and parents) cope with a life-threatening disease, Duchene muscular dystrophy. The child doesn't know he is sick yet. Does...

24 March 2020 2,046 3 View

Does anyone has experience with NEB "time-saver enzyme" overnight digestion?

Hi all, I used the Thermo-ANZA series for overnight digestion of plasmid backbone to ensure clean digestion without any problem. How about NEB? The website said all NEB "time-saver enzyme"...

24 March 2020 7,636 2 View

Difference between cell culture plates and flasks?

In the lab we always use culture plates (33, 60, 100, 150mm) for passing cells and conducting all sorts of experiments. I wonder what is the perks of using cell culture plates v.s. flasks? Should...

12 March 2020 1,361 2 View

Why do my 2D orthogonal cutting forces in abaqus spike down to zero instead of staying fairly steady as shown by others examples?

In every simulation I've ran, the cutting forces spike up and down instead of staying at a fairly constant value. Why is this happening? I've defined a rigid body reference point on the cutting...

23 January 2020 9,059 3 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

How to derive equations for a resonant buck converter with ZVS?

I am not understanding how each author is deriving the equations and the working principle. Individual authors are giving their own working principle explanation for different circuit topology....

12 February 2021 496 1 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View