I need a concrete and already tested and approved protocol for DNA digestion using nuclease P1 and alkaline phosphatase. We're going to measure oxidation in DNA bases using a standard kit, but prior to the procedure we have to digest DNA into separate nucleosides. Have any of you applied such a protocol before? Can you recommend any protocols or articles to look into?
Does anyone know the amount of the nuclease P1 and Alkaline phosphatase I would need to add to digest DNA?
Hi
If you try ( by your self) the provided company protocol and start with well known sample first as a positive control if it is work then move to your sample. if its not, that means the product dose not work.
Thank you Mohammed.
I would especially need the amount/ units of enzyme to be used.
Hi Nikhita Shankar , I am also going to measure oxidation damage using
DNA Damage ELISA Kit (8-OHdG). I was wondering if you found any standard protocol for DNA digestion using nuclease P1 and alkaline phosphatase.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology