How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

Does anyone knows about a really easy but still comprehensive ImageJ tutorial?

I am new to this software. I am in need of analyzing images from ocular ultrasounds (black/white) and find a way to quantify the internal reflectivities from the vitreous cavity in cases of...

16 February 2021 4,238 2 View

How do I delete a publication that I added?

I added two times the same thing by mistake and I need to remove one. Thanks.

11 January 2021 6,118 5 View

How can I identify the points that are poking out from a point cloud plot?

I have a cloud plot of data relating two important variables for an ecological analysis. I was defining the "abnormal class" as the data points that are >=20 for the Y-axis and >=20 for the...

22 September 2020 7,679 16 View

All-against-all pairwise global alignments?

Hi all, I have a collection of nucleotide sequences corresponding to one functional domain of a hallmark gene of viruses. So in principle I am working with relatively-homogeneous functional...

28 March 2020 8,869 3 View

When do you want to use full protein sequences or only conserved domains to make a phylogenetic tree?

Hi, I'd like to know what difference makes using FULL PROTEIN SEQUENCES versus PROTEIN CONSERVED DOMAIN SEQUENCES in order to make a phylogenetic tree. In my case, the goal is to put in...

23 April 2019 3,090 4 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

20 December 2018 3,718 3 View

Does anyone know any fishery of poecilidae (poeciliidae) that is captured for human consumption?

I am making a description of a fishery of poecilids from estuarine systems of Guatemala, specifically Poecilia butleri. I would like to know if in any other part of the world, poecilids are...

11 December 2018 351 1 View

How determine Fermi level for a metal in Tight Binding model?

Dear all, I have already all the information of my tight binding result, but we have a lot of theoretical arguments to believe that the Fermi level is in another level. We know that the most...

28 November 2018 7,964 3 View

How can I prepare a Bacterial cell pellet for TEM and SEM observations?

I am looking for a brief method to prepare bacteria pellets forTEM and SEM analysis. Thanks in advance

05 June 2018 2,509 0 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How can I import .sav file into LISREL?

I've just downloaded free trial version of LISREL 10.3 but I got stuck with importing .sav (IBM SPSS 25.) file. I mainly followed the steps of this video...

15 February 2021 752 2 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,306 3 View

How to screen phytochemicals directly from the crude plant latex?

Number of methods are available to screen phytochemicals through extract prepared in different solvents including aqueous and organic. Is there any method/ published work related to phytochemical...

01 February 2021 7,348 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How is it possible to rank variables using Likert scale?

Hello Everyone, I have a questionnaire of a 3-point Likert scale for an overall evaluation for a service, and several detailed attributes evaluation of that service. For example: Overall...

31 January 2021 9,612 8 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View

Best tool to build missing residues, minimize and validate a ligand model?

I am working on a ligand that is co-crystalized to parotein, but the ligand is missing some residues that makes it appear as if it was separated into two ligands! what I need is to connect them...

23 January 2021 3,975 7 View