How to report a post-hoc comparison for robust mixed ANOVA?

Hi all I have conducted a robust mixed design ANOVA using WRS2 in R. My data are time taken in seconds, and not normally distributed. I cannot remove outliers as the experiment is based around...

23 July 2020 6,766 4 View

One possible process for implementing environmental sustainability?

Can the process be named and list 3 phases of how it would be implemented

14 July 2020 6,249 11 View

How do I produce a collimated image "beam" in my optical system?

I am trying to design an optical system to produce stereo images of an object with a single camera. To do this, I am going to utilize three mirrors: a reflector at each stereo angle and a...

13 July 2020 1,532 4 View

Can someone translate this paragraph into plain English?

Hi all, I'm looking into observational studies to try and better characterize treatment-resistant depression. I'm having trouble deciphering the paragraph in bold below, having read it multiple...

19 February 2020 9,823 2 View

What can you tell me about an E.coli strain named Lemo21 from NEB?

I am currently trying to express human tyrosinase in E.coli and as you know this enzyme being a membrane protein it comes with a fair share of difficulties relating to expression in BL21(DE3)....

03 October 2018 6,996 3 View

How can I prepare a Bacterial cell pellet for TEM and SEM observations?

I am looking for a brief method to prepare bacteria pellets forTEM and SEM analysis. Thanks in advance

05 June 2018 2,509 0 View

Does anyone know of a working antibody against Orexin-A/Hypocretin-1 for immunofluorescence in Brain & Spinal Cord?

Our working antibody (https://www.scbt.com/scbt/product/orexin-a-antibody-c-19) has been discontinued, and we are looking for a replacement. We have tried 3 other antibodies (see below) to...

14 January 2018 9,040 5 View

Protein purification protocol?

Hello fellow scientists, here i have a bit of a seemingly demoralizing issue with protein purification. Could you please help? I have attached a commassie image for clarity. 10mM imidazole, 50 mM...

25 December 2017 3,853 4 View

Where can I find a Mini Sleep Questionnaire?

I am looking for the Zoomer J et al Mini Sleep Questionnaire for screening large populations

10 September 2017 8,588 3 View

Help with FACS voltage setting for FSC v SSC?

I am new to flow cytometry and have not had formal training on setting appropriate voltage / (gain?) values. I ran PBMCs isolated from whole blood, and tried to adjust FSC and SSC values so all my...

01 August 2017 1,188 4 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View