How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What are the solvent requirement to study protein conformational changes via NMR?

I wanted to study the conformational changes taking place in gamma D crystallin (eye lens protein) at low pH via NMR. What would be the preferred solvent requirements for this particular protein...

31 December 2020 2,103 4 View

Why does using lens (achromatic doublet lens) for imaging detection onto camera affect magnification in Interferometric Scattering (iSCAT) microscopy?

Hello every scientific explorers!! Recently, I have been interested in Interferometric Scattering (iSCAT) microscopy, the scattering-based imaging microscopy. One of the problems I am facing is...

27 December 2020 8,092 2 View

How can I set to thin film on the substrate in ZEMAX?

My research is about 3D-printing add nanoparticles attach to copper. So I'm trying to use ZEMAX to calculate absorption, someone knows how to set the thin film on ZEMAX? Have any suggestions or...

26 November 2020 7,200 1 View

What were the impacts of liberalization and globalization on Russia in the decade following the collapse of the Soviet Union?

I am writing an essay that will examine the negative impacts of globalization by criticizing the neoliberal paradigm through the lens of Russia during the 1990s.

19 November 2020 5,122 4 View

How to measure the point spread function of confocal microscopy using 405 or 440 laser lines?

100- 200 nm fluorescent beads are available from suppliers but only excited at UV (360 nm) or green and red range (i.e., >488 nm). Also. If the objective lens to be evaluated have (NA

18 November 2020 2,096 3 View

No title

17 November 2020 6,197 5 View

How best to Collimate an LED to feed into a series of fibers?

I am currently using a simple 8mm focal length lens from Carlco to focus my light from a single small LED into a bundle of fibers that have a total diameter of 9mm. The problem I am having is...

11 November 2020 5,332 5 View

I am working on Lensless Microscopy. Can anybody tell me how to get spectrum of a sample in Fresnel region without using any lens??

I want to get the spectrum of an object illuminated by an incoherent source at the CMOS sensor without using any lens.

01 November 2020 225 2 View

How to measure the focusing efficiency of Fresnel zone plate (FWHM is in micrometer scale) ?

We want to measure the focusing efficiency of the Fresnel zone plate with a very small FWHM. "The focusing efficiency is defined as the ratio of the total power in a circular aperture with a...

01 November 2020 1,252 7 View

In microscopy why "smaller the aperture, greater the depth of field"?

12 October 2020 3,636 2 View