What is (response surface graph)?

For optimization of reaction conditions, most of the time we have several factors to take care of, like pH, temp, initial conc., ionic strength... etc. How we can express in a professional way our...

27 May 2020 3,150 3 View

For a hydrothermal autoclave, can we use a glass container instead of the teflon?

To increase the temperature of the autoclave higher than 160 or 200 C, we need thermally stable materials as the reaction container. So what kind of materials can be used??

17 May 2020 8,963 7 View

Can I use matlab deep learning toolbox for regression problems?

Hi everybody I want to use MATLAB deep learning toolbox to relate the activity (pIC50) of molecules to their molecular descriptors. Can I use convolutional neural network with a regression layer...

03 February 2020 1,874 6 View

How to determine the degree of similarity between two signals?

I'v been doing an investigation about detection of a specific signal and I need to determine that how a received signal resembles the reference signal. A primary approach to this problem is done...

06 November 2017 6,369 7 View

How we can apply FRP in Concrete beam with Abaqus?

Hello all, I want to modelling a Concrete Beam with ABAQUS, My intention is that first beam cracked and then the FRP, apply to beam and analysis continue. can you help me?

11 January 2017 8,962 6 View

How can we determine representative volume element length (Lrve) for concrete?

for periodic materials, determining Lrve is simple but for a non-homogeneous material such as concrete, it is not that simple. any advice such as methods, references, etc. would be appreciated.

04 November 2016 6,044 3 View

How can i do a meta-analysis of a subject in sport and physical education?

I want to learn about this type of research.

11 January 2014 9,954 3 View

The internal energy change for isothermal processes is zero. But for the vaporization of liquid (which is an isothermal process) it is not zero.

The internal energy change for isothermal processes is known to be equal to zero. But in the case of liquid vaporization (e.g. liquid water to steam) (boiling at constant temperature), the...

01 January 1970 7,917 4 View

Why some Cr(III) complexes have labile reactions ??

01 January 1970 7,787 7 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How can I import .sav file into LISREL?

I've just downloaded free trial version of LISREL 10.3 but I got stuck with importing .sav (IBM SPSS 25.) file. I mainly followed the steps of this video...

15 February 2021 752 2 View

How to screen phytochemicals directly from the crude plant latex?

Number of methods are available to screen phytochemicals through extract prepared in different solvents including aqueous and organic. Is there any method/ published work related to phytochemical...

01 February 2021 7,348 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View

Best tool to build missing residues, minimize and validate a ligand model?

I am working on a ligand that is co-crystalized to parotein, but the ligand is missing some residues that makes it appear as if it was separated into two ligands! what I need is to connect them...

23 January 2021 3,975 7 View

Does the initial cell number play a role in affecting the output variables like IC50, EC50, etc.in cell-based drug screening assays? If so, how?

While performing cell-based assays to assess drug toxicity or drug efficacy, how is the initial cell seeding density determined? What is the standard followed in the pharmaceutical industry while...

07 January 2021 6,983 3 View

Can i use MM-PBSA to calculate the binding energy of a glycan ligand? ad how?

I have a protein-ligand (glycan) complex and want to do an MM-PBSA analysis using AMBERTOOLS after doing MDS (NAMD). What are the files necessary I should prepare for glycan? Note: I used...

30 December 2020 2,658 2 View

Is there any online webserver for DNA-ligand docking?

I have used Autodock 4.2 for docking studies and trying haddock for DNA-Ligand docking. It shows an error and not supported DNA PDB file. Suggest a template for DNA and ligand PDB file and also...

29 December 2020 1,322 8 View